A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat

J. G. R. Ufkes; M. Ottenhof; R. C. Aalberse

doi:10.1016/0160-5402(83)90036-0

A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat

J. G. R. Ufkes^*
, M. Ottenhof
, R. C. Aalberse

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, Ppl, Fres, Cdyn, and Ri) and cardiovascular function (BP and Fheart) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8-10 min) bron-choconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21-150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo. © 1983.

Original language	English
Pages (from-to)	175-181
Journal	Journal of pharmacological methods
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0160-5402(83)90036-0
Publication status	Published - 1983
Externally published	Yes

Access to Document

10.1016/0160-5402(83)90036-0

Cite this

@article{25b2388241884c84b88f2c0ae2565777,

title = "A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat",

abstract = "Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, Ppl, Fres, Cdyn, and Ri) and cardiovascular function (BP and Fheart) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8-10 min) bron-choconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21-150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo. {\textcopyright} 1983.",

author = "Ufkes, \{J. G. R.\} and M. Ottenhof and Aalberse, \{R. C.\}",

year = "1983",

doi = "10.1016/0160-5402(83)90036-0",

language = "English",

volume = "9",

pages = "175--181",

journal = "Journal of pharmacological methods",

issn = "0160-5402",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat

AU - Ufkes, J. G. R.

AU - Ottenhof, M.

AU - Aalberse, R. C.

PY - 1983

Y1 - 1983

N2 - Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, Ppl, Fres, Cdyn, and Ri) and cardiovascular function (BP and Fheart) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8-10 min) bron-choconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21-150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo. © 1983.

AB - Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, Ppl, Fres, Cdyn, and Ri) and cardiovascular function (BP and Fheart) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8-10 min) bron-choconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21-150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo. © 1983.

UR - https://www.scopus.com/pages/publications/0020564282

UR - https://www.ncbi.nlm.nih.gov/pubmed/6876809

U2 - 10.1016/0160-5402(83)90036-0

DO - 10.1016/0160-5402(83)90036-0

M3 - Article

C2 - 6876809

SN - 0160-5402

VL - 9

SP - 175

EP - 181

JO - Journal of pharmacological methods

JF - Journal of pharmacological methods

IS - 3

ER -

A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat

Abstract

Access to Document

Other files and links

Fingerprint

Cite this