A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

He-Ling Wang; Jianying Zhang; Shu-qin Cao; Maria Jose Lagartos-Donate; Shi-qi Zhang; Sofie Lautrup; Zeping Hu; Costas A. Lyssiotis; Riekelt H. Houtkooper; Evandro F. Fang

doi:10.1016/j.xpro.2024.103428

A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

He-Ling Wang
, Jianying Zhang
, Shu-qin Cao
, Maria Jose Lagartos-Donate
, Shi-qi Zhang
, Sofie Lautrup
, Zeping Hu
, Costas A. Lyssiotis
, Riekelt H. Houtkooper
, Evandro F. Fang^*

^*Corresponding author for this work

University of Oslo
Central South University
Tsinghua University
University of Michigan, Ann Arbor
University of Amsterdam
Amsterdam UMC
The Norwegian Centre on Healthy Ageing (NO-Age) and the Norwegian National Anti-Alzheimer's Disease (NO-AD) Networks

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.

Original language	English
Article number	103428
Journal	STAR Protocols
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2024.103428
Publication status	Published - 20 Dec 2024
Externally published	Yes

Keywords

Cell Biology
Metabolism
Molecular Biology
Neuroscience

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title = "A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood",

abstract = "Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.",

keywords = "Cell Biology, Metabolism, Molecular Biology, Neuroscience",

author = "He-Ling Wang and Jianying Zhang and Shu-qin Cao and Lagartos-Donate, \{Maria Jose\} and Shi-qi Zhang and Sofie Lautrup and Zeping Hu and Lyssiotis, \{Costas A.\} and Houtkooper, \{Riekelt H.\} and Fang, \{Evandro F.\}",

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doi = "10.1016/j.xpro.2024.103428",

language = "English",

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T1 - A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

AU - Wang, He-Ling

AU - Zhang, Jianying

AU - Cao, Shu-qin

AU - Lagartos-Donate, Maria Jose

AU - Zhang, Shi-qi

AU - Lautrup, Sofie

AU - Hu, Zeping

AU - Lyssiotis, Costas A.

AU - Houtkooper, Riekelt H.

AU - Fang, Evandro F.

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.

AB - Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.

KW - Cell Biology

KW - Metabolism

KW - Molecular Biology

KW - Neuroscience

UR - https://www.scopus.com/pages/publications/85208031833

U2 - 10.1016/j.xpro.2024.103428

DO - 10.1016/j.xpro.2024.103428

M3 - Article

C2 - 39487980

SN - 2666-1667

VL - 5

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 103428

ER -

A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

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