4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices

Pierre N. E. de Graan; Loes H. Schrama; Arjen B. Brussaard; Reinhard Jork; Willem Hendrik Gispen

doi:10.1016/0006-8993(87)91392-8

4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices

Pierre N. E. de Graan
, Loes H. Schrama
, Arjen B. Brussaard
, Reinhard Jork
, Willem Hendrik Gispen

Utrecht University
Institute of Pharmacology and Toxicology, Germany

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.

Original language	English
Pages (from-to)	345-349
Journal	Brain research
Volume	404
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(87)91392-8
Publication status	Published - 24 Feb 1987
Externally published	Yes

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title = "4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices",

abstract = "Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86\%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). {\textcopyright} 1987.",

author = "\{de Graan\}, \{Pierre N. E.\} and Schrama, \{Loes H.\} and Brussaard, \{Arjen B.\} and Reinhard Jork and Gispen, \{Willem Hendrik\}",

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language = "English",

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T1 - 4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices

AU - de Graan, Pierre N. E.

AU - Schrama, Loes H.

AU - Brussaard, Arjen B.

AU - Jork, Reinhard

AU - Gispen, Willem Hendrik

PY - 1987/2/24

Y1 - 1987/2/24

N2 - Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.

AB - Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.

UR - https://www.scopus.com/pages/publications/0023155583

UR - https://www.ncbi.nlm.nih.gov/pubmed/3032338

U2 - 10.1016/0006-8993(87)91392-8

DO - 10.1016/0006-8993(87)91392-8

M3 - Article

C2 - 3032338

SN - 0006-8993

VL - 404

SP - 345

EP - 349

JO - Brain research

JF - Brain research

IS - 1-2

ER -

4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices

Abstract

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